Immobilized Penicillin G Amidase Enzyme catalyzes the hydrolysis of the amidic bond as well as used in the kinetic resolution, amide coupling in beta lactams. It is used for β-lactam Antibiotics synthesis and also for producing intermediates like 6 APA, 7 ADCA, 7 AVCA etc.
Penicillin G acylase (PGA) is one of very important industrial enzymes used in the production of polysynthetic beta-lactam antibiotics. This enzyme catalyzes the production of amoxicillin by acylation of silylated 6-aminopenicillanic acid with the appropriate acid chloride hydrochloride the efficiency of the process and the purity of the product are increased.
Cefoxitin is a second-generation cephamycin antibiotic developed from Cephamycin C. It was synthesized in order to create an antibiotic with a broader spectrum. Cephalexin is produced from 7-amino desacetoxy cephalosporanic acid and phenylglycine ester by enzymatic acylation with acylating enzyme i.e. Penicillin G amidase.
Esterase for Cefuroxime Axetil synthesis
Cefuroxime Axetil is a second generation semi-synthetic cephalosporin and a beta-lactam antibiotic with bactericidal activity.
Cefoxitin a broad-spectrum cepha antibiotic, it is derived from cephamycin C. The bactericidal action of cefoxitin results from inhibition of cell wall synthesis. Cefoxitin has in vitro activity against a wide range of gram-positive and gram-negative organisms.
The transesterification chemical reaction is base catalyzed. Any strong base capable of deprotonating the alcohol for e.g. NaOH, KOH, and Sodium methoxide can be used. The use of lipases enzyme makes the reaction less sensitive to high free fatty-acid content, which is a problem during standard biodiesel process. Another problem with the lipase reaction is that methanol cannot be used as it inactivates the lipase catalyst after one batch. However, if methyl acetate is used, the lipase is not inactivated and can be used for several batches, making the lipase system much more cost effective.